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dna sequencing service  (Fasmac Co Ltd)

 
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    Fasmac Co Ltd dna sequencing service
    Dna Sequencing Service, supplied by Fasmac Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+sequence+service/pm42309546-67-20-23?v=Fasmac+Co+Ltd
    Average 86 stars, based on 1 article reviews
    dna sequencing service - by Bioz Stars, 2026-07
    86/100 stars

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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus <t>DNA</t> ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense <t>sequence</t> (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).
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    (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

    Journal: Bio-protocol

    Article Title: Plasmid Curing of Pseudoalteromonas haloplanktis TAC125 Using Homologous Recombination and PTasRNA Gene Silencing

    doi: 10.21769/BioProtoc.5687

    Figure Lengend Snippet: (A) 1.2% agarose gel electrophoresis of the PCR fragment and the digested pB40-79-PTasRNA lon vector. MW = 1 kb Plus DNA ladder; 1 = asRNA repB PCR fragment (110 bp); 2 = Xho I-pB40-79-PT- Pst I linear fragment (4,979 bp); 3 = pB40-79-PTasRNA lon plasmid DNA (negative control). ( B ) Map of the PTasRNA vector. The diagram illustrates the modular organization of the silencing vector. The cloning site designed for the antisense sequence (pink) is flanked by Pst I and Xho I restriction sites located between the two paired termini sequences (in gray). The vector includes two origins of replication: oriC for the propagation of the plasmid in E. coli strains (in red) and oriR for propagation in Ph TAC125 (in green); oriT is pivotal for the conjugative transfer (in orange); Ph LacR encodes the regulator of p LacZ promoter (not shown); amp R represents the ampicillin resistance marker (in blue).

    Article Snippet: DNA sequencing service (e.g., Eurofins Genomics, Ebersberg, Germany) 7.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Cloning, Sequencing, Marker